The mask shown up on the MASK panel may be edited as described in a previous section.
Segmentation Method Selection
At this point, the CLUSTERING (K MEANS) segmentation method has to be selected in the lower right. The panel below the selection then shows the parameters to be configured, namely the Clustering of the Average or All input frames, the Number of clusters as well as the Number of iterations during which the clusters are adjusted.
Note that all pixels within the mask are assigned to one of the clusters, and the process doesn't include any kind of neighborhood relation. The classification is solely based on the signal time course in each pixel.
To start the k-means segmentation please activate the Segmentation action button. The required processing time depends on several factors, particularly the number of iterations, and may take several minutes. The segmentation result is finally shown on the SEGMENTS panel.